Bcl-2 (2B11) Monoclonal Antibody

Applications

  • WB
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-50256M
Product Name Bcl-2 (2B11) Monoclonal Antibody
Applications WB, IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human protein purified from E.coli (His-Bcl-2)
Clonality Monoclonal
Clone # 2B11
Isotype IgG1 κ
Concentration 2ug/ul
Purification Ammonium sulfate precipitation
Storage HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Store for 1 year at -20°C from date of shipment.
Target
Gene ID 596
Swiss Prot P10415
Synonyms Bcl-2; PPP1R50; Apoptosis regulator Bcl-2; BCL2
Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Application Dilution
WB 1:300-1000
IP 1-2ug