MAP2 (9C4) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP2 (159CT34.12.3.4) Monoclonal Antibody (bsm-51036M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP2 (159CT34.12.3.4) Monoclonal Antibody (bsm-51036M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P
IF

Reactivity

Human

Overview
Catalog #	bsm-51036M
Product Name	MAP2 (9C4) Monoclonal Antibody
Applications	WB, IHC-P, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MAP2 Monoclonal antibody was raised using purified His-tagged recombinant human MAP2.
Clonality	Monoclonal
Clone #	9C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4133
Swiss Prot	P11137
Synonyms	MAP2A; MAP2B; MAP2C; Microtubule-associated protein 2; MAP-2; MAP2
Background	The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Application Dilution
WB	1:500-2000
IHC-P	1:100-500
IF	1:100-500

Applications

Reactivity

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