Cdk8 (4A4) Monoclonal Antibody

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-52032R
Product Name Cdk8 (4A4) Monoclonal Antibody
Applications WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Cdk8 protein, full length.
Clonality Monoclonal
Clone # 4A4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID 1024
Swiss Prot P49336
Synonyms Cyclin-dependent kinase 8; CDK8
Background Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Application Dilution
WB 1:1000-2000