Cyclin D3 (4A8) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-52047R
Product Name Cyclin D3 (4A8) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Cyclin D3 protein, around C-terminal 200aa.
Clonality Monoclonal
Clone # 4A8
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID 896
Swiss Prot P30281
Synonyms G1/S-specific cyclin-D3; CCND3
Background Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Application Dilution
WB 1:1000-2000
IF(ICC) 1:50-200