Cytokeratin 2e (2F7) Monoclonal Antibody

Human skin lysates probed with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .

$Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed and paraffin-embedded Human skin cancer tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human skin cancer tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human esophagus tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human esophagus tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 2e (2F7) Monoclonal Antibody, Unconjugated (bsm-52061R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-52061R
Product Name	Cytokeratin 2e (2F7) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cytokeratin 2e protein, around 350-500aa.
Clonality	Monoclonal
Clone #	2F7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3849
Swiss Prot	P35908
Synonyms	K2e; KRTE; CK-2e; KRT2A; KRT2E; Keratin, type II cytoskeletal 2 epidermal; Cytokeratin-2e; Epithelial keratin-2e; Keratin-2 epidermis; Keratin-2e; Type-II keratin Kb2; KRT2
Background	Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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