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IRF2 (5F2) Monoclonal Antibody

Human lung lysates probed with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Human lung lysates probed with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A549 cells were stained with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A549 cells were stained with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52115R
Product Name IRF2 (5F2) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human IRF2 protein, around C-terminal 100aa.
Clonality Monoclonal
Clone # 5F2
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3660
Swiss Prot P14316
Synonyms IRF-2; Interferon regulatory factor 2; IRF2
Background Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200

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