B Raf (Tyr401) (2B3) Monoclonal Antibody

$Lane 1: Mouse testis; Lane 2: PC-12 lysate probed with B-Raf (Tyr401) (2B3) Monoclonal Antibody (bsm-52135R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.$

Lane 1: Mouse testis; Lane 2: PC-12 lysate probed with B-Raf (Tyr401) (2B3) Monoclonal Antibody (bsm-52135R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.

PC-3 cells were stained with B-Raf (Tyr401) (2B3) Monoclonal Antibody (bsm-52135R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52135R
Product Name	B Raf (Tyr401) (2B3) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide derived from human B Raf(T401), around 350-450aa (phospho T401).
Modification Site	Tyr401
Clonality	Monoclonal
Clone #	2B3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months..
Target
Gene ID	673
Swiss Prot	P15056
Synonyms	NS7; BRAF1; RAFB1; B-RAF1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF
Background	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Application Dilution
WB	1:300-1000
IF(ICC)	1:50-200

Applications

Reactivity

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