Smad2 (9A3) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-52223R
Product Name Smad2 (9A3) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Smad2 protein, around N-terminal 1-200aa.
Clonality Monoclonal
Clone # 9A3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID 4087
Swiss Prot Q15796
Synonyms JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2
Background Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Dilution
WB 1:300-1000
IF(ICC) 1:50-200