25% Off Flow Cytometry Antibodies - Use Code: "EggyIggy2024" - Valid 3/29-4/5/24 for U.S. customers only

PCK1 (6C1) Monoclonal Antibody

Applications

  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-54230R
Product Name PCK1 (6C1) Monoclonal Antibody
Applications FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic peptide within human PCK1 aa 1-50
Clonality Monoclonal
Clone # 6C1
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 5105
Swiss Prot P35558
Subcellular location Cytoplasm
Synonyms Phosphoenolpyruvate carboxykinase cytosolic [GTP]; phosphoenolpyruvate carboxykinase 1; PEPCK-C; PCK1; PEPCK1.
Background Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.
Application Dilution
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200