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CHAF1A (1D4) Monoclonal Antibody

Lane 1: K562 Cells; Lane 2: A431 Cells; Probed with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: K562 Cells; Lane 2: A431 Cells; Probed with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Applications

  • WB
  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-54414R
Product Name CHAF1A (1D4) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human p150 CAF1 aa 100-300.
Clonality Monoclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 10036
Swiss Prot Q13111
Subcellular location Nucleus
Synonyms Chromatin assembly factor 1 subunit A; Chromatin assembly factor I p150 subunit; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; hp150; p150 CAF1
Background Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP 1-2ug

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