Phospho-FAK (Y397) Monoclonal Antibody

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

$N2A cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

N2A cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

$NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-FAK (Y397)) Monoclonal Antibody, Unconjugated (bsm-54549R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54549R
Product Name	Phospho-FAK (Y397) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein
Modification Site	Tyr397
Clonality	Monoclonal
Clone #	1G2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5747
Swiss Prot	Q05397
Subcellular location	Cytoplasm, Nucleus, Cell membrane, Cell junction
Synonyms	FADK 1 antibody, FADK antibody, FAK related non kinase polypeptide antibody, FAK1 antibody, FAK1_HUMAN antibody, Focal adhesion kinase 1 antibody, Focal adhesion Kinase antibody, Focal adhesion kinase isoform FAK Del33 antibody, Focal adhesion kinase related nonkinase antibody, FRNK antibody, p125FAK antibody, pp125FAK antibody, PPP1R71 antibody, Protein phosphatase 1 regulatory subunit 71 antibody, Protein tyrosine kinase 2 antibody, Protein-tyrosine kinase 2 antibody, Ptk2 antibody, PTK2 protein tyrosine kinase 2 antibody
Background	This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200

Applications

Reactivity

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