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SLC22A3 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC22A3 Monoclonal Antibody, Unconjugated (bsm-54709R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC22A3 Monoclonal Antibody, Unconjugated (bsm-54709R) at 1:50 for 30 minutes at room temperature, DAB staining.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC22A3 Monoclonal Antibody(bsm- R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green).
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC22A3 Monoclonal Antibody(bsm- R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green).

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-54709R
Product Name SLC22A3 Monoclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic Peptide within C terminal human SLC22A3/OCT3.
Clonality Monoclonal
Clone # 7H7
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 6581
Swiss Prot O75751
Subcellular location Membrane
Synonyms EMT antibody, EMT organic cation transporter 3 antibody, EMTH antibody, Extraneuronal monoamine transporter antibody, OCT 3 antibody, OCT3 antibody, Orct 3 antibody, Orct3 antibody, Organic cation transporter 3 antibody, S22A3_HUMAN antibody, SLC22 A3 antibody, SLC22A 3 antibody, Slc22a3 antibody, Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody, Solute carrier family 22 (organic cation transporter) member 3 antibody, Solute carrier family 22 member 3 antibody
Background Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

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