MSH2 Monoclonal Antibody

ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Monoclonal", "Clone Number"=>"4H8", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-54721R", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436", "Host"=>"Rabbit", "images"=>"[{\"id\":\"36376\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"18748\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:50\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 30min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated (bsm-54721R) at 1:50 for 30 minutes at room temperature, DAB staining.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":\"1\",\"sample_tissue_id\":\"6\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"18748\\/36376_raw.jpg\",\"filepath_med\":\"18748\\/36376_med.jpg\",\"filepath_thumb\":\"18748\\/36376_thumb.jpg\",\"display_order\":\"1\",\"lock_version\":\"1\",\"created_at\":\"2021-01-06 01:28:07\",\"updated_at\":\"2021-01-06 01:28:07\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/36376\"},{\"id\":\"37272\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"18748\",\"productconjugation_id\":null,\"application_id\":\"3\",\"application_note\":\"\",\"dilution\":\"1:50\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Hela cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MSH2 Monoclonal Antibody(bsm-54721R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).\",\"sample_type_id\":\"1\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":\"13\",\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"18748\\/37272_raw.jpg\",\"filepath_med\":\"18748\\/37272_med.jpg\",\"filepath_thumb\":\"18748\\/37272_thumb.jpg\",\"display_order\":\"2\",\"lock_version\":\"1\",\"created_at\":\"2021-04-07 02:48:44\",\"updated_at\":\"2021-04-07 02:48:44\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/37272\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"ET7110-50", "Product URL"=>"https://www.biossusa.com/products/bsm-54721R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"Recombinant fragment within C terminal human MSH2.", "Storage"=>"Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.", "Storage Buffer"=>"Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.", "Storage Condition"=>"Store at -20°C for 12 months.", "Subcellular Locations"=>"Nucleus, Chromosome", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246", "Synonyms"=>"BAT26 antibody, COCA 1 antibody, COCA1 antibody, DNA mismatch repair protein Msh2 antibody, FCC 1 antibody, FCC1 antibody, hMSH2 antibody, HNPCC 1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, MSH 2 antibody, Msh2 antibody, MSH2_HUMAN antibody, MutS homolog 2 antibody, MutS homolog 2 colon cancer nonpolyposis type 1 antibody, MutS protein homolog 2 antibody", "Target Protein"=>"MSH2"} -->