{"product_id":"ihc0410h","title":"Human NGF Ready-To-Use IHC Kit","description":"\u003ch1 data-mce-fragment=\"1\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\n\u003cb data-mce-fragment=\"1\"\u003eHuman NGF Ready-To-Use IHC Kit\u003c\/b\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\n\u003c\/h1\u003e\n\u003cb data-mce-fragment=\"1\"\u003eCat. No.: \u003c\/b\u003eIHC0410H\u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSample Type: \u003c\/b\u003eFFPE tissue \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSize: \u003c\/b\u003e50T (including a control slide) \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eStorage and Stability: \u003c\/b\u003ePlease store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eBackground\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eNerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGFhave shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eSynonyms\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eMaterials Supplied\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.791%;\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 7%;\"\u003eNumber\u003c\/th\u003e\n\u003cth style=\"width: 42%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003e50T\u003c\/th\u003e\n\u003cth style=\"width: 20%;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePBS Buffer (powder)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e2L x 2\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e20x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eAntigen Retrieval Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e20ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e100x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eEndogenous Peroxidase Blocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003eRTU\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eBlocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e5\u003c\/td\u003e\n\"\u003ctd style=\"\" width:\u003ePrimary Antibody \n(Human NGF Recombinant Rabbit mAb)\u003c\/td\u003e\"\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e6\u003c\/td\u003e\n\"\u003ctd style=\"\" width:\u003eSecondary Antibody \n(Goat Anti-Rabbit IgG H\u0026amp;L, HRP conjugated)\u003c\/td\u003e\"\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component A\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component B\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e-20°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eCounter Staining Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e10 \u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDifferentiation Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eMounting Media\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eControl slide (human cerebellum)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 slide\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDatasheet\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eNotes\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eThe positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eDo not allow slides to dry at any time during this procedure. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePlease don't replace the matching reagents in this product with other manufacturers' products. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eAs DAB is a carcinogen, please take necessary precautions. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\n\u003cbr\u003eAs DAB is a carcinogen, please take necessary precautions.\n\u003cbr\u003e\u003cbr\u003eImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"50 Test","offer_id":50489881461035,"sku":"IHC0410H","price":499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/IHC0410H_1_ce86fa58-2ee5-4fe5-9b26-d6430accbfb1.png?v=1745604118","url":"https:\/\/www.biossusa.com\/products\/ihc0410h","provider":"Bioss","version":"1.0","type":"link"}