{"product_id":"ihc0443h","title":"Human MtTF1 Ready-To-Use IHC Kit","description":"\u003ch1 data-mce-fragment=\"1\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\n\u003cb data-mce-fragment=\"1\"\u003eHuman MtTF1 Ready-To-Use IHC Kit\u003c\/b\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\n\u003c\/h1\u003e\n\u003cb data-mce-fragment=\"1\"\u003eCat. No.: \u003c\/b\u003eIHC0443H\u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSample Type: \u003c\/b\u003eFFPE tissue \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSize: \u003c\/b\u003e50T (including a control slide) \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eStorage and Stability: \u003c\/b\u003ePlease store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eBackground\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eMitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, andcontribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eSynonyms\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eAI661103; Hmgts; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; tsHMG; MTDPS15.Validation Data\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eMaterials Supplied\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.791%;\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 7%;\"\u003eNumber\u003c\/th\u003e\n\u003cth style=\"width: 42%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003e50T\u003c\/th\u003e\n\u003cth style=\"width: 20%;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePBS Buffer (powder)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e2L x 2\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e20x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eAntigen Retrieval Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e20ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e100x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eEndogenous Peroxidase Blocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003eRTU\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eBlocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e5\u003c\/td\u003e\n\"\u003ctd style=\"\" width:\u003ePrimary Antibody \n(Human MtTF1 Recombinant Rabbit mAb)\u003c\/td\u003e\"\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e6\u003c\/td\u003e\n\"\u003ctd style=\"\" width:\u003eSecondary Antibody \n(Goat Anti-Rabbit IgG H\u0026amp;L, HRP conjugated)\u003c\/td\u003e\"\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component A\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component B\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e-20°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eCounter Staining Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e10 \u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDifferentiation Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eMounting Media\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eControl slide (human kidney)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 slide\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDatasheet\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eNotes\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eThe positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eDo not allow slides to dry at any time during this procedure. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePlease don't replace the matching reagents in this product with other manufacturers' products. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eAs DAB is a carcinogen, please take necessary precautions. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\n\u003cbr\u003eAs DAB is a carcinogen, please take necessary precautions.\n\u003cbr\u003e\u003cbr\u003eImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"50 Test","offer_id":50489888178475,"sku":"IHC0443H","price":499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/IHC0443H_1_5a71d8fe-4561-4110-aed0-d03bb3f179dd.png?v=1745604138","url":"https:\/\/www.biossusa.com\/products\/ihc0443h","provider":"Bioss","version":"1.0","type":"link"}