{"product_id":"ihc0512h","title":"Human HIF-1 beta Ready-To-Use IHC Kit","description":"\u003ch1 data-mce-fragment=\"1\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\n\u003cb data-mce-fragment=\"1\"\u003eHuman HIF-1 beta Ready-To-Use IHC Kit\u003c\/b\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\n\u003c\/h1\u003e\n\u003cb data-mce-fragment=\"1\"\u003eCat. No.: \u003c\/b\u003eIHC0512H\u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSample Type: \u003c\/b\u003eFFPE tissue \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSize: \u003c\/b\u003e50T (including a control slide) \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eStorage and Stability: \u003c\/b\u003ePlease store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eBackground\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eHIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eSynonyms\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eARNT; Arnt1; BHLHE2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714; bHLHe2.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eMaterials Supplied\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.791%;\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 7%;\"\u003eNumber\u003c\/th\u003e\n\u003cth style=\"width: 42%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003e50T\u003c\/th\u003e\n\u003cth style=\"width: 20%;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePBS Buffer (powder)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e2L x 2\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e20x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eAntigen Retrieval Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e20ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e100x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eEndogenous Peroxidase Blocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003eRTU\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eBlocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePrimary Antibody (Human HIF-1 beta Recombinant Rabbit mAb)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eSecondary Antibody (Goat Anti-Rabbit IgG H\u0026amp;L, HRP conjugated)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component A\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component B\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e-20°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eCounter Staining Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e10 \u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDifferentiation Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eMounting Media\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eControl slide (Human testis)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 slide\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDatasheet\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eNotes\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eThe positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eDo not allow slides to dry at any time during this procedure. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePlease don't replace the matching reagents in this product with other manufacturers' products. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eAs DAB is a carcinogen, please take necessary precautions. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\n\u003cbr\u003eAs DAB is a carcinogen, please take necessary precautions.\n\u003cbr\u003e\u003cbr\u003eImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"50 Test","offer_id":51224239472939,"sku":"IHC0512H","price":499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/IHC0512H_1_9e5dc398-ee14-4be7-9754-3ff5b02a443e.png?v=1756831827","url":"https:\/\/www.biossusa.com\/products\/ihc0512h","provider":"Bioss","version":"1.0","type":"link"}