{"product_id":"ihc0603r","title":"Rat PSMA1 Ready-To-Use IHC Kit","description":"\u003ch1 data-mce-fragment=\"1\" style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\n\u003cb data-mce-fragment=\"1\"\u003eRat PSMA1 Ready-To-Use IHC Kit\u003c\/b\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\n\u003c\/h1\u003e\n\u003cb data-mce-fragment=\"1\"\u003eCat. No.: \u003c\/b\u003eIHC0603R\u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSample Type: \u003c\/b\u003eFFPE tissue \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eSize: \u003c\/b\u003e50T (including a control slide) \u003cb data-mce-fragment=\"1\"\u003e\u003cbr\u003eStorage and Stability: \u003c\/b\u003ePlease store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eBackground\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003eProteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately31), barrel-shaped molecular machine with an apparent molecular weight of approximately2. 5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eSynonyms\u003c\/b\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eMaterials Supplied\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.791%;\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 7%;\"\u003eNumber\u003c\/th\u003e\n\u003cth style=\"width: 42%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003e50T\u003c\/th\u003e\n\u003cth style=\"width: 20%;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"width: 12%;\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePBS Buffer (powder)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e2L x 2\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e20x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eAntigen Retrieval Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e20ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e100x\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eEndogenous Peroxidase Blocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003eRTU\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eBlocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003ePrimary Antibody (Rat PSMA1 Recombinant Rabbit mAb)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eSecondary Antibody (Goat Anti-Rabbit IgG H\u0026amp;L, HRP conjugated)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e2-8°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component A\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eChromogen Component B\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e0.3ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003e-20°C\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eCounter Staining Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e10 \u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDifferentiation Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e6ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10%;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eMounting Media\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eControl slide (Rat brain)\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 slide\u003c\/td\u003e\n\u003ctd style=\"width: 10%; text-align: center;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eRTU\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7%;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"width: 43%;\"\u003eDatasheet\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 10%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb data-mce-fragment=\"1\"\u003eNotes\u003cbr\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eThe positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eDo not allow slides to dry at any time during this procedure. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePlease don't replace the matching reagents in this product with other manufacturers' products. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003eAs DAB is a carcinogen, please take necessary precautions. \u003c\/b\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cb data-mce-fragment=\"1\"\u003ePBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\n\u003cbr\u003eAs DAB is a carcinogen, please take necessary precautions.\n\u003cbr\u003e\u003cbr\u003eImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"50 Test","offer_id":51224244879659,"sku":"IHC0603R","price":499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/IHC0603R_1_090e3582-a6e2-410f-ba06-1e5658ab90be.png?v=1756831852","url":"https:\/\/www.biossusa.com\/products\/ihc0603r","provider":"Bioss","version":"1.0","type":"link"}