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HDAC8 (4C3) Monoclonal Antibody

Lane 1: Hela; Lane 2: K562 lysate probed with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: K562 lysate probed with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HepG2 cells were stained with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were stained with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

  • WB
  • IHC-P
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52088R
Product Name HDAC8 (4C3) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human HDAC8 protein, around N-terminal 150aa.
Clonality Monoclonal
Clone # 4C3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 55869
Swiss Prot Q9BY41
Synonyms HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1; Histone deacetylase 8; HDAC8
Background Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

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