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His Tag Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Tags
  • Others
Overview
Catalog # bs-10582R-Biotin
Product Name His Tag Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Tags, Others
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from His Tag
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Synonyms HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500