Dnmt3a (8A1) Monoclonal Antibody

$Lane 1: Hela cells; Lane 2: Human brain; Lane 3: Human heart ; Probed with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells; Lane 2: Human brain; Lane 3: Human heart ; Probed with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Dnmt3a (8A1) Monoclonal Antibody (bsm-52453R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52453R
Product Name	Dnmt3a (8A1) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein human Dnmt3a between 600-700 amino acids
Clonality	Monoclonal
Clone #	8A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1788
Swiss Prot	Q9Y6K1
Synonyms	DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA, Dnmt3a, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A
Background	Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200