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Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163\/M130 Polyclonal Antibody, Unconjugated( bs-2527R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163\/M130 Polyclonal Antibody, Unconjugated (bs-2527R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163\/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37\u00b0C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37\u00b0C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \\n

Catalog #1: bs-2527R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Catalog #1: bs-0647R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.
Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1329R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Human U937 cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:20 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
U937 lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0195R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-0081R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0081r-100ul

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated(bs-3330R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PERK (Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PERK(Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Mouse spleen cells probed with \\tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1019R

Applications: WB, FCM, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1495R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with a secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812r-TR

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cells labeled with Anti-VEGF Polyclonal Antibody (bs-0279R).
Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 23,46kDa
HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\\n

Catalog #1: bs-0279r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse myocardium labeled with (bs-1074R) Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size:66kD.\\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1074r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti BRN3A Polyclonal Antibody, Unconjugated (bs-3669R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3669R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody

Catalog #1: bs-2013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:800 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse liver lysates probed (bs-0127R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 21kDa.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining

Catalog #1: bs-0127R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-P-selectin Polyclonal Antibody (bs-0561R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: K562 cell lysates; Lane 2: Huvec cell lysates; Lane 3: Jurkat cell lysates probed with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0561r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-GLUT4 Polyclonal Antibody (bs-0384R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Anti-GLUT4 Polyclonal Antibody (bs-0384R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Tissue\/cell: NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0384R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti-NGN3\/Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval with 0.4% pepsin at 37\u00b0C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Liver; Probed Neurogenin 3, unconjugated (bs-0922R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Breast Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-0922R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:300 followed by conjugation to a secondary antibody and staining.
Lane 1: Mouse Testis lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Testis lysates; Lane 4: Rat Cerebrum lysates probed with GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0654R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4563R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti Smad3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Ovary lysates; Lane 3: HT1080 cell lysates; Lane 4: Jurkat cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2\/3 Polyclonal Antibody(bs-3484R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Skin lysates; Lane 5: Mouse Kidney lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Kidney lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human A549 cell lysates; Lane 11: Human Hela cell lysates; Lane 12: Human HT1080 cell lysates; Lane 13: Human A431 cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3484R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-LAMP2\/CD107B Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2379R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0216R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: DU145 cell lysates; Lane 2: LoVo cell lysates; probed with E-Selectin\/CD62E Polyclonal Antibody, Unconjugated (bs-1273R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1273R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with AMPK alpha-1\/2 (Thr183\/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HepG2 cell lysates probed with phospho-AMPK alpha-2 (Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebral cortex lysates probed with AMPK alpha-1\/2 (Thr183\/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1\/2 (Thr183\/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4002R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human glioma labeled with Anti-GLI1\/ZFP5 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1206R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PXR Polyclonal Antibody, Unconjugated (bs-2334R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Mouse Liver lysates probed with PXR Polyclonal Antibody, Unconjugated (bs-2334R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PXR (bs-2334R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2334R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse heart lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Mouse Ovary; Probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-8996R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

RSC96 cells probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R ) at 0.2ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with\\tCD9\/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-2489R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4595R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal cancer labeled with Anti-\u03b2-catenin\/Catenin \u03b2 Polyclonal Antibody, Unconjugated (bs-1165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Beta catenin Polyclonal Antibody, Unconjugated(bs-1165R) at 1:200 followed by conjugation to the secondary antibody,
Formalin-fixed and paraffin embedded rat skin labeled with Anti-\u03b2-catenin\/Catenin \u03b2 Polyclonal Antibody, Unconjugated (bs-1165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-\u03b2-catenin\/Catenin \u03b2 Polyclonal Antibody, Unconjugated(bs-1165R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-1165R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAP2\/MAP-2A.B.C Polyclonal Antibody, Unconjugated (bs-1369R) followed by conjugation to the secondary antibody and DAPI staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1369R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor\/CD142\/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor\/CD142\/F3 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band
Image provided by the Independent Validation Program,badge number 029589:Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD142 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Human A431 cell lysates; Lane 4: Human MDA-MB-231 cell lysates; Lane 5: Human SiHa cell lysates probed with Tissue factor Polyclonal Antibody, Unconjugated (bs-4690R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4690R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2527R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody,FITC Conjugated(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2527R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.\\n
Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Kindey lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1658R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program (badge number 29749). Lane 1: A549 cell extract Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (bs-0588R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0588R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon labeled with Anti SLC26A4 Polyclonal Antibody, Unconjugated (bs-6787R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human TT cell lysates; Lane 2: Human U251 cell lysates probed with SLC26A4 Polyclonal Antibody, Unconjugated (bs-6787R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6787R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti Phospho-Smad3(Ser423\/425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
Formalin-fixed and paraffin embedded human liver carcinoma labeled with Rabbit Anti-Smad3 (Ser423 + Ser425) Polyclonal Antibody (bs-3425R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser423 + Ser425) Polyclonal Antibody(bs-3425R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Ser423 + Ser425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3425R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti CMKLR1 Polyclonal Antibody, Unconjugated (bs-12478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Huvec (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMKLR1 Antibody (bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Liver tissue lysates probed with CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-10185R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin Receptor Polyclonal Antibody(bs-0109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0109R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates\uff1bLane 2: Mouse Lung lysates probed with AKT1\/2\/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33033m

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bs-0468R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: FITC

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (bs-0036R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0036R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10011R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated