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Catalog #1: bsm-33124M-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

TT lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A431 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach tissue probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-17422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-17422R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-17422R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Histone H3.3 (7B12) Monoclonal Antibody (bsm-52110R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3.3 (7B12) Monoclonal Antibody, Unconjugated (bsm-52110R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3.3 (7B12) Monoclonal Antibody, Unconjugated (bsm-52110R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3.3 (7B12) Monoclonal Antibody, Unconjugated (bsm-52110R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52110r

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HL60 cell lysates probed with Histone H4 (Acetyl K12) Polyclonal Antibody, Unconjugated (bs-3746R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H4 (Acetyl K12) Antibody(bs-3746R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H4 (Acetyl K12)) Polyclonal Antibody, Unconjugated (bs-3746R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H4 (Acetyl K12)) Polyclonal Antibody, Unconjugated (bs-3746R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3746R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3746R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3746R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Piotr Mamczur as part of the free sample program. Immunofluorescent localization of acetylated histone H4 in mouse squamous cell cancer (KLN-205 cell line) with BS-4707R (1:20) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Histone H4 (Acetyl K16) Antibody(bs-4707R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-4707R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4707R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4707R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H4 (Acetyl K5) Polyclonal Antibody, Unconjugated (bs-10721R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human MCF-7 cell lysates probed with Histone H4 (Acetyl K5) Polyclonal Antibody, Unconjugated (bs-10721R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Histone H4 (Acetyl K5)) polyclonal Antibody, Unconjugated (bs-10721R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10721R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-10721R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-10721R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human MCF-7 cell lysates probed with Histone H4 (Acetyl K8) Polyclonal Antibody, Unconjugated (bs-4016R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Histone H4 (Acetyl K8)) polyclonal Antibody, Unconjugated (bs-4016R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Histone H4 (Acetyl K8) Antibody(bs-4016R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-4016R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-4016R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-4016R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-3826R

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0239r-100ul

Applications: ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-0239r-biotin-100ul

Applications: ELISA

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bs-0239r-hrp-100ul

Applications: ELISA

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bs-4981R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-4981R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-4981R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: HRP

Catalog #1: bs-4980R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-4980R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bs-4980R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bsm-72242m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72243m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72246m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72244m

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72247m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72248m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72249m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-4982R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-4982R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-4982R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: HRP

Catalog #1: bs-4942R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-4942R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-4942R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: HRP

Catalog #1: bs-6968R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-HLA-E Polyclonal Antibody (bs-1279R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-HLA-E Polyclonal Antibody (bs-1279R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Raji cell lysates probed with Rabbit Anti-HLA E Polyclonal Antibody (bs-1279R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1279R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1279R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-8604R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8604R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-8604R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-10251R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated