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Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54\/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54\/ICAM-1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 60kD. Observed band size: 60kD.
Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Lane 1: Hela cell lysates; Lane 2: Hela KO \r\nICAM1 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0608R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52466r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52466r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-52466r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (ascites of bsm-10697M) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-10697m

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10697m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-ICAM-2\/CD102 Polyclonal Antibody, Unconjugated (bs-1258R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-ICAM-2\/CD102 Polyclonal Antibody, Unconjugated (bs-1258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1258R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1258R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1258R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-62574r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62574r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-62574r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-1821R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

K562 lysates probed with ICAM4 Polyclonal Antibody, Uconjugated (bs-7637R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Mouse bone lysates probed with ICAM4 Polyclonal Antibody, unconjugated (bs-7637R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ICAM4 Polyclonal Antibody(bs-7637R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-7637R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICK Polyclonal Antibody, Unconjugated (bs-15536R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICK Polyclonal Antibody, Unconjugated (bs-15536R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-15536R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-15536R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-15536R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

BxPC-3 Cells (Human pancreas adenocarcinoma) were fixed with 4% PFA (10min at room temperature). The cells were then incubated in 10% goat serum to block non-specific protein-protein interactions for 20 min at 37\u00b0C. Then the cells were probed with ICMT Polyclonal Antibody, Unconjugated (bs-8019R) at 1:200 for 60 minutes at room temperature followed by incubation with a Goat Anti-Rabbit IgG, Cy5 Conjugated (bs-0295G-Cy5) secondary antibody at a 1:200 dilution for 40 minutes at 37\u00b0C. DAPI (5ug\/ml; blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with ICMT Polyclonal Antibody, Unconjugated (bs-8019R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-8019R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-8019R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-8019R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2583R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2583R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2583R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-15537R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-15537R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-15537R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3529R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U-2OS cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Hela cell lysates probed with Id1 (5F1) Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ID1) monoclonal Antibody, Unconjugated (bsm-54101R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ID1 Monoclonal Antibody(bsm-54101R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ID1 Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54101r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54101r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-54101r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-3515R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3515R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-3515R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-6229R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6229R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-6229R-Biotin

Applications: IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-6229R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-6229R-HRP

Applications: IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-6669R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6669R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-6669R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IDE) polyclonal Antibody, Unconjugated (bs-0018M) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IDE Antibody(bs-0018M)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0018m

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human gastric carcinoma labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated (bs-0018R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-IDE Polyclonal Antibody, Unconjugated (bs-0018R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IDE Polyclonal Antibody, Unconjugated (bs-0018R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Muscle lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Stomach lysates; Lane 5: Rat Liver lysates probed with IDE Polyclonal Antibody, Unconjugated (bs-0018R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0018R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0018R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0018R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

25 ug total protein per lane of various lysates (see on figure) probed with IDE monoclonal antibody, unconjugated (bsm-61379R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IDE Monoclonal Antibody, Unconjugated (bsm-61379R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IDE Monoclonal Antibody, Unconjugated (bsm-61379R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IDE Monoclonal Antibody, Unconjugated (bsm-61379R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61379r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61379r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin