CD40L (4C2) Monoclonal Antibody

Flow cytometric analysis of thp-1 cells with CD40L (4C2) Monoclonal Antibody(bsm-52939R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Hela cells lysates; Lane 2: Mouse liver cells lysates; Probed with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells lysates; Lane 2: Mouse liver cells lysates; Probed with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52939R
Product Name	CD40L (4C2) Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	N terminus human CD40L
Clonality	Monoclonal
Clone #	4C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	959
Swiss Prot	P29965
Synonyms	CD40 ligand, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40-L, TRAP, CD40LG
Background	Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD4L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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