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Catalog #1: bs-42386R-BF488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-42386R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-42386R-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-42386R-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-42386R-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-42386R-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-42386R-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-42386R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-42386R-APC

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bs-42386R-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-42386R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-42386R-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-42386R-BF405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-42386R-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-42386R-BF555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-42386r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-42386R-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

4% Paraformaldehyde-fixed Hela (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (bs-42326R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HepG2 (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (bs-42326R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bs-42326r

Applications: ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-42189r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-42189r-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-42189r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-42189r-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-42189r-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-42189r-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bs-42189r-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-42189r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-42189r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-42189r-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-42189r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-42189r-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-42189r-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-42189r-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-42189r-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-42189r-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-42189r-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-42189r-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-42189r-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-42189r-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-42189r-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-42189r-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-42189r-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-42189r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-42189r-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-42189r-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bs-42189R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-FCGR1A\/CD64 antibody (bs-41395R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Blank control\uff08black line\uff09:Raw264.7. \r\n \r\nPrimary Antibody (green line): Rabbit Anti-CD64 antibody (bs-41395R) \r\n\r\nDilution:1ug\/Test; \r\n \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-rabbit IgG-AF488\r\n\r\nDilution: 0.5ug\/Test. \r\n\r\nIsotype control\uff08orange line\uff09: Normal Rabbit IgG\r\n\r\nProtocol\r\n\r\nThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bs-41395r

Applications: Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3644R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25848r-pe-100ul

Applications: IF

Reactivity: Human

Conjugate: PE