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25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, unconjugated (bsm-10889M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Monoclonal Antibody, Unconjugated (bsm-10889M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Monoclonal Antibody, Unconjugated (bsm-10889M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-10889m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-10889m-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-30178a

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-10889m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-43662R-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-10889m-bf350-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-10889m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-49172r-percp-cy7-100ul

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-49172R-BF555

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-49172R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-10889m-apc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-43662R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-49172R-PerCP

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-49172R-PE-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-43662R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-43662R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-10889m-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-49172R-PE-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-43662R-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-49172R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-43662R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-10889m-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-49172R-BF750

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-49172R-BF350

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-49172R-Cy3

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-49172R-APC-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-49172R-BF488

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-49172R-PE-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-49172R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-49172R-Cy5

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-43662R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-49172R-Cy5.5

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-49172R-Cy7

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-49172R-BF594

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-43662R-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-49172R-BF680

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-49172R-APC

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bs-43662R-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-43662R-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-49172R-APC-Cy5.5

Applications: WB, IF

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-43662R-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-43662R-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-43662R-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-43662R-PerCP

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-43662R-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-43662R-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-43662R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43662R-BF594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594