Human IL-18 ELISA Kit

Principle of the Assay

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-18 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-18 present is bound by the immobilized antibody and the detection antibody specific for IL-18 is binds to the combination of capture antibody-IL-18 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-18 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-18 sample concentration determined.

For Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.

Target Information

Interleukin 18 (IL-18) is an 18 kDa cytokine which is identified as a costimulatory factor for production of interferon-r (IFN-r) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1b converting enzyme (ICE, caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from dendritic cells, activated macrophages, Kupffer cells, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalons. IFN-r is produced by activated T and NK cells and plays critical roles in the defense against microbial pathogens. IFN-g activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens expression, and inhibits osteoclast activation. IL-18 acts on T helper 1-type (Th1) cells, and in combination with IL-12 strongly induces production of IFN-g by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN-g and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-g in T cells, enhancement of Fas ligand expression by Th1 cells.

GENE ID	3606
SWISS PROT	Q14116
SYNONYMS	IGIF; IL-18; IL-1g; IL1F4

Materials Supplied

Kit Components	96 Wells Quantity/Size
Aluminium pouches with a Microwell Plate coated with antibody to human IL-18 (812)	1 plate
Human IL-18 standard lyophilized, 2500 pg/ml upon reconstitution	2 vials
Concentrated Biotin-Conjugate anti-human IL-18 antibody	2 vials
Streptavidin-HRP solution	2 vials
Standard /sample Diluent	1 bottle
Biotin-Conjugate antibody Diluent	1 bottle
Streptavidin-HRP Diluent	1 bottle
Wash Buffer Concentrate 20x (PBS with 1% Tween-20)	1 bottle
Substrate Solution	1 vial
Stop Solution	1 vial
Adhesive Films	4 pieces
Product data sheet	1 copy

Storage

Store at 2 - 8°C

Performance Characteristics

REPEATABILITY	The coefficient of variation of both intra-assay and inter-assay were less than 10%.
SENSITIVITY	The minimum detectable dose was 20pg/mL.
ASSAY RANGE	39 - 2500 pg/mL
SPECIFICITY	This assay recognizes both natural and recombinant human IL-18. The factors listed below were prepared at 50ng/ml in standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

Factors assayed for cross-reactivity

Recombinant human	IFN-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F
Recombinant mouse	IL-17

Data Analysis Assistance

We have partnered with MyAssays to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by MyAssays specifically for BSKH1056.

https://www.myassays.com/bioss-human-il-18-elisa-kit.assay