Human S100B ELISA Kit
Principle of the Assay
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for S100B has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any S100B present is bound by the immobilized antibody and the detection antibody specific for S100B is binds to the combination of capture antibody-S100B in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of S100B present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven S100B standard dilutions and S100B sample concentration determined.
For Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
S100B belongs to the S100 subgroup of the EF-hand family of calcium binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.
|SYNONYMS||NEF; S100; S100-B; S100beta|
|Kit Components||96 Wells Quantity/Size|
|Aluminium pouches with a Microwell Plate coated with antibody to human S100B (812)||1 plate|
|Human S100B standard lyophilized, 6000 pg/ml upon reconstitution||2 vials|
|Concentrated Biotin-Conjugate anti-human S100B antibody||2 vials|
|Streptavidin-HRP solution||2 vials|
|Standard /sample Diluent||4 bottle|
|Biotin-Conjugate antibody Diluent||1 bottle|
|Streptavidin-HRP Diluent||1 bottle|
|Wash Buffer Concentrate 20x (PBS with 1% Tween-20)||1 bottle|
|Substrate Solution||1 vial|
|Stop Solution||1 vial|
|Adhesive Films||4 pieces|
|Product data sheet||1 copy|
|Storage||Store at 2 - 8°C|
|REPEATABILITY||The coefficient of variation of both intra-assay and inter-assay were less than 10%.|
|SENSITIVITY||The minimum detectable dose was 46pg/mL.|
|ASSAY RANGE||93.75 - 6000 pg/mL|
|SPECIFICITY||This assay recognizes both natural and recombinant human S100B. The factors listed below were prepared at 50ng/ml in standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.|
Factors assayed for cross-reactivity
|Recombinant human||NDR1, p53, RAGE/Fc Chimera, S100A6, S100A4|
Data Analysis Assistance
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