Mouse IL-1ß ELISA Kit

Principle of the Assay

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1β has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-1β present is bound by the immobilized antibody and the detection antibody specific for IL-1β is binds to the combination of capture antibody- IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-1β present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-1β sample concentration determined.

For Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.

Target Information

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1αand IL-1β, which are the products of distinct genes, but which recognize the same cell surface receptors. With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, IL-1 is not produced by the cells of healthy individuals. However, in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen.

IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Intracellular IL-1βconsists exclusively of the 31 kDa precursor form. Extracellular IL-1βconsists of a mixture of both unprocessed and mature IL-1β. These results indicate that processing takes place subsequently to secretion and is not tightly coupled to secretion. The specific protease apparently responsible for the processing of IL-1β, designated interleukin-1β-converting enzyme (ICE), has been described.

IL-1 possesses a wide variety of biological activities. IL-1 also plays an important role in immune functions, having effects on macrophages / onocytes, Tlymphocytes, B lymphocytes, NK cells, and LAK cells. It acts on macrophages/ monocytes, inducing its own synthesis as well as the production of TNF and IL-6. It activates T cells, resulting in IL-2 production and expression of IL-2 receptors. IL-1 also induces the production of GM-CSF and IL-4 from activated T cells. It induces B cell proliferation and maturation and increased immunoglobulin synthesis.

GENE ID 16176
SWISS PROT P10749
SYNONYMS Il-1b; IL-1beta

Materials Supplied

Kit Components 96 Wells Quantity/Size
Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-18 (812) 1 plate
Mouse IL-1β standard lyophilized, 2000 pg/ml upon reconstitution 2 vials
Concentrated Biotin-Conjugate anti-mouse IL-1β antibody 2 vials
Streptavidin-HRP solution 2 vials
Standard /sample Diluent 1 bottle
Biotin-Conjugate antibody Diluent 1 bottle
Streptavidin-HRP Diluent 1 bottle
Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle
Substrate Solution 1 vial
Stop Solution 1 vial
Adhesive Films 4 pieces
Product data sheet 1 copy

Storage

Storage Store at 2 - 8°C

Performance Characteristics

REPEATABILITY The coefficient of variation of both intra-assay and inter-assay were less than 10%.
SENSITIVITY The minimum detectable dose was 7pg/mL.
ASSAY RANGE 31.25-2000 pg/mL
SPECIFICITY This assay recognizes both natural and recombinant mouse IL-1β. The factors listed below were prepared at 50ng/ml in standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Factors assayed for cross-reactivity
Recombinant mouse IL-1α, IL-1ra, IL-1 RI, IL-1 RII
Recombinant porcine IL-1β

 

Data Analysis Assistance

We have partnered with MyAssays to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by MyAssays specifically for BSKM1015.

https://www.myassays.com/bioss-mouse-il-1%ce%b2-elisa-kit.assay