Human Calbindin Ready-To-Use IHC Kit
Human Calbindin Ready-To-Use IHC KitCat. No.: IHC0106H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.
CAB27; CALB 1; CALB; CALB1; Calbindin 1 28kDa; Calbindin-D-28K; Calbindin D28; D 28K; D28K; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D dependent calcium binding protein avian-type; Vitamin D-dependent calcium-binding protein; CALB1_HUMAN.
|1||PBS Buffer (powder)||2L x 2||20x||RT|
|2||Antigen Retrieval Buffer||20ml||100x||2-8°C|
|3||Endogenous Peroxidase Blocking Buffer||3ml||RTU||2-8°C|
|5||Primary Antibody (Human Calbindin Rabbit pAb)||6ml||RTU||2-8°C|
|6||Secondary Antibody (HRP-Goat anti-Rabbit IgG pAb)||6ml||RTU||2-8°C|
|7||Chromogen Component A||0.3ml||RTU||-20°C|
|8||Chromogen Component B||0.3ml||RTU||-20°C|
|9||Counter Staining Reagent||5ml||RTU||RT|
|12||Control slide (Human kidney)||1 side||RTU||RT|
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
Please cite this product as "IHC0106H, Bioss Antibodies".
Citation example: “Human tissue sections using Human Calbindin IHC Kit (IHC0106H, Bioss Antibodies) were stained for Calbindin according to the manufacturer's instructions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.