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Rabbit IgG Isotype Control, PerCP Conjugated

APPLICATIONS

    • WB
 
    • FCM
 
    • IF(IHC-P)
 
    • IF(IHC-F)
 
    • IF(ICC)
OVERVIEW
CATALOG # bs-0295P-PerCP
PRODUCT NAME Rabbit IgG Isotype Control, PerCP Conjugated
APPLICATIONS WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
SPECIFICATIONS
CONJUGATION PerCP 
HOST Rabbit
CLONALITY Polyclonal
ISOTYPE IgG
CONCENTRATION 1ug/ul
PURIFICATION
STORAGE BUFFER Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
STORAGE CONDITION Store at -20°C for 12 months. 
TARGET
BACKGROUND Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.
APPLICATION DILUTION
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
CITATIONS
Ma, Hongwei, et al. "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." ACS applied materials & interfaces 2.11 (2010): 3223-3230.
PubMed
Huang, Mo, et al. "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." Colloids and Surfaces B: Biointerfaces 85.1 (2011): 92-96.
PubMed
Lee, Ju Yeon, et al. "Molecular cloning and expression analysis of pig CD7." Veterinary Research Communications (2014): 1-7.
  • PIG
PubMed
Zhuang, Huiyu, et al. "Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells." Biochimie (2014).
PubMed
Brestoff, Jonathan R., et al. "Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity." Nature (2015).
  • MOUSE
PubMed
Wu, Qiong, et al. "A highly sensitive SPR biosensor based on a graphene oxide sheet modified with gold bipyramids, and its application to an immunoassay for rabbit IgG." Microchimica Acta (2015): 1-8.
  • OTHER
PubMed
Fan, Kequan, et al. "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.
  • OTHER
PubMed
Wu, Yuanzi, Hongwei Ma, and Dayong Gu. "Quartz Crystal Microbalance as a tool for biomolecular interaction study." RSC Advances (2015).
  • OTHER
PubMed
Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863
  • IHC-P
  • MOUSE
PubMed
Shang, Luoran, et al. "Photonic Crystal Microbubbles as Suspension Barcodes." Journal of the American Chemical Society (2016).
  • OTHER
PubMed
Zhou, S. M., et al. "Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2 mutant of Haemophilus parasuis SC096 strain via the classical complement pathway." The Veterinary Journal 196.1 (2013): 111-113.
  • OTHER
  • OTHERS
PubMed
Benesch, Matthew GK, et al. "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." The FASEB Journal 29.9 (2015): 3990-4000.
  • IHC-P
  • HUMAN
PubMed
Benesch, Matthew GK, et al. "Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer." Endocrine-related cancer 22.4 (2015): 593-607.
  • IHC-P
  • HUMAN
PubMed
Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).
  • OTHER
PubMed
Wang, Huimin, et al. "Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma." Oncotarget (2016).
  • IP
  • HUMAN
PubMed
Zhang, Jie, et al. "Role of PTX3 in corneal epithelial innate immunity against Aspergillus fumigatus infection." Experimental Eye Research (2016).
  • IHC-P
PubMed
Christensen, Anders, et al. "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." Oncotarget.
  • FCM
  • HUMAN
PubMed
Miyabe, Yoshishige, et al. "Complement C5a receptor is the key initiator of neutrophil adhesion igniting immune complex–induced arthritis." Science Immunology 2.7 (2017): eaaj2195.
  • IHC-P
  • MOUSE
PubMed
Chen, Baiwen, et al. "Effects of 1, 25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." International Immunopharmacology 47 (2017): 182-189.
  • IHC-F
  • MOUSE
PubMed
Jiang, Wen-Shuai, et al. "Reduced graphene oxide-based optical sensor for detecting specific protein." Sensors and Actuators B: Chemical (2017).
  • OTHER
PubMed
Xia, Wenlong, and Jianwei Jiao. "Histone variant H3. 3 orchestrates neural stem cell differentiation in the developing brain." Cell Death & Differentiation (2017).
PubMed
Wang, Zhaojun, et al. "Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/-mice as well as smooth muscle cells." Vascular (2017): 1708538117713395.
  • IHC-P
  • MOUSE
PubMed
Zhao, Shuai, et al. "Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology." Proceedings of the National Academy of Sciences (2017): 201704155.
  • OTHER
PubMed
Braun, Molly, et al. "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." Brain, Behavior, and Immunity (2017).
PubMed
Hao, Yingying, et al. "c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer." Oncology Reports.
  • IHC-P
PubMed
Shen, Tianjin, et al. "Brain-specific deletion of histone variant H2A. z results in cortical neurogenesis defects and neurodevelopmental disorder." Nucleic Acids Research.
  • IHC
PubMed
Deng et al. Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma. (2015) J.Exp.Clin.Cancer.Re. 34:96
  • IHC-P
  • HUMAN
PubMed
Cao et al. Leptin promotes migration and invasion of breast cancer cells by stimulating IL-8 production in M2 macrophages. (2016) Oncotarge. 7:65441-65453
  • IHC
  • MOUSE
PubMed
Wang et al. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. (2017) J.Diabetes.Res. 2017:1428537
  • OTHER
  • MOUSE
PubMed
Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234
  • FCM
  • HUMAN
PubMed
Forsberg et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. (2016) Elife. 5
  • IHC
  • MOUSE
PubMed
Zhuang et al. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. (2014) Mol.Cancer. 13:243
  • IHC
  • HUMAN
PubMed
Shen et al. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. (2018) Nucleic.Acids.Res. 46:2290-2307
  • IF
  • MOUSE
PubMed
Du YQ et al. A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI.(2018) Journal of Materials Chemistry B.
  • OTHER
Chen X et al.Dual-digital encoded suspension array based on Raman spectroscopy and laser induced breakdown spectroscopy for multiplexed biodetection.(2019)Sensors and Actuators B: Chemical, 282, 457–468.
  • OTHER
  • RABBIT
"Tanaka M et al. Effect of mirabegron on tight junction molecules in primary cultured rat Sertoli cells. Andrologia. 2019 Jan 31:e13241. "
  • IF(ICC)
PubMed
Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.
  • OTHER
PubMed
Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019.
  • OTHER
Peng T et al. The interaction of LOXL2 with GATA6 induces VEGFA expression and angiogenesis in cholangiocarcinoma. Int J Oncol. 2019 Jul 15.
  • OTHER
PubMed
Wang B et al. Gold-nanorod-enhanced Raman spectroscopy encoded micro-quartz pieces for the multiplex detection of biomolecules. Anal Bioanal Chem. 2019 Jul 6.
  • OTHER
PubMed
Saribas GS et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman's syndrome. Acta Histochem. 2019 Nov 24:151465.
  • IHC-P
PubMed
Jiang Y et al. Transcriptome profile of bovine iPSCs derived from Sertoli Cells. Theriogenology. 2019 Nov 19. pii: S0093-691X(19)30522-9.
  • FCM
PubMed
Liu Y et al. IGFBP2 promotes immunosuppression associated with its mesenchymal induction and FcγRIIB phosphorylation in glioblastoma. PLoS One. 2019 Sep 27;14(9):e0222999. 
  • FCM
  • IHC-P
PubMed
Liu X et al. Polydopamine-based molecular imprinted optic microfiber sensor enhanced by template-mediated molecular rearrangement for ultra-sensitive C-reactive protein detection. Chemical Engineering Journal. 2020, May 387: 124074.
  • OTHER
  • OTHER SPECIES
"Wang R et al. Expression of 5T4 extracellular domain fusion protein and preparation of anti-5T4 monoclonal antibody with high affinity and internalization efficiency.Protein Expr Purif. 2018 Jul 5. pii: S1046-5928(18)30212-2. "
  • FCM
  • IHC-P
  • OTHERS

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