PCNA Recombinant Antibody, HRP Conjugated

$25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035R-HRP) at 1:1000 dilution and 4\u00b0C overnight incubation.$

25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035R-HRP) at 1:1000 dilution and 4\u00b0C overnight incubation.

$Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human\r\n Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human\r\n Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33035r-hrp-100ul
Product Name	PCNA Recombinant Antibody, HRP Conjugated
Applications	WB, IHC-P, IHC-F
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	HRP
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PCNA
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	5111
Swiss Prot	P12004
Synonyms	Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen
Application Dilution
WB	1:5000-20000
IHC-P	IHC-P1:200-1000
IHC-F	IHC-F1:200-1000

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