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NEURO10 Applicable Antibodies



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Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-0081R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0081r-100ul

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 23kD. Observed band size: 23kD.
Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1024R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated(bs-1992R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (bs-1992R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Phospho-FRAP1\/mTOR(Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 followed by conjugation to the secondary antibody and DAB stainin
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with mTOR (Ser2448) Polyclonal Antibody(bs-3494R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3494R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-UCHL1\/PGP9.5 Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3806R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates, L2 rat brain lysates probed : Anti-GDF9 (bs-2951R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 48kDa and 62kD
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2951R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0061R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: HRP

Catalog #1: bs-0756R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0061R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Hsc70 Polyclonal Antibody(bs-5117R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Hsc70) polyclonal Antibody, Unconjugated (bs-5117R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5117R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Catalog #1: bs-0756R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0756R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0756R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-0756R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-0756R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2951R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-0756R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-0756R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-1992R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE

Catalog #1: bs-3806R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: FITC

Catalog #1: bs-3806R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Cy3

Catalog #1: bs-3494R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3494R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2951R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-1992R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bs-0756R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0061R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: FITC

Catalog #1: bs-0061R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5

Catalog #1: bs-0061R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy3

Catalog #1: bs-0061R-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: RBITC

Catalog #1: bs-0061R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0061R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP

Catalog #1: bs-0061R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy7

Catalog #1: bs-0061R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5.5

Catalog #1: bs-0061R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5

Catalog #1: bs-0061R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy7

Catalog #1: bs-0061R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy5.5

Catalog #1: bs-0061R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC

Catalog #1: bs-0061R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-750

Catalog #1: bs-0061R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-680

Catalog #1: bs-0061R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-647

Catalog #1: bs-0061R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-594

Catalog #1: bs-0061R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-555

Catalog #1: bs-0061R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-488

Catalog #1: bs-0061R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-405

Catalog #1: bs-0061R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-350