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Catalog #1: bs-3067R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3067R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-3067R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3069R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-3069R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rabbit

Conjugate: HRP

Catalog #1: bs-3069R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rabbit

Conjugate: Biotin

Catalog #1: bs-3071R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3071R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-3071R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-3072R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3073R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-3073R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3073R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was used at a 100 fold (A and B) and 500 fold (C and D) dilution. A no primary antibody negative control (E and F) was used to verify the specificity of the stain. The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. \r\nImages in the upper row (A, C, and E) were captured at 10x magnification, while the images below (B, D, and F) were taken at 20x magnification. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program. \r\n
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MCSF Receptor (Tyr723) Polyclonal Antibody(bs-3077R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3077R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3077R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-3077R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-MCSF Receptor(Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Hela cell lysates probed with MCSF Receptor (Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raw264.7 cell lysates probed with MCSF Receptor (Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CSF1R (Tyr923) Antibody\uff08bs-3079R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-3079R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3079R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3079R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Antigen: bs-3080P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: rat brain lysates Lane 2: human gastric carcinoma lysates probed with Anti phospho-CYLD(Ser418)Polyclonal Antibody, Unconjugated (bs-3080R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 105kD. Observed band size: 105kD.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYLD (Ser418) Polyclonal Antibody, Unconjugated (bs-3080R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3080R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3080R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-3080R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-3081R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CaMK2 (Tyr230) Polyclonal Antibody(bs-3081R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3081R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3081R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3084R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
293T cell lysates probed with Beta-Catenin (Ser33 + Ser37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with beta Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3084R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3084R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3087R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-3085R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3087R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3087R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-3089R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3089R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-3089R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-3090R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3090R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-3090R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-3091R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Antigen: bs-3091P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-CDK1 (Thr14)) polyclonal Antibody, Unconjugated (bs-3091R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-CDK1 (Thr14) Polyclonal Antibody(bs-3091R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Thymus tissue lysates; Lane 4: Rat Thymus tissue lysates ; Lane 5: Human SH-SY5Y cell lysates probed with Phospho-CDK1 (Thr14) Polyclonal Antibody, Unconjugated (bs-3091R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3091R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3091R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Anti Phospho-cdc2 (Tyr15) Polyclonal Antibody, Unconjugated (bs-3092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Phospho-CDK1 (Tyr15) Polyclonal Antibody, Unconjugated (bs-3092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3092R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3092R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-3092R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-3093R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3093R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-3093R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Mouse Embryo lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2: Human MCF-7 lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-cdc25A (Ser76) Antibody(bs-3094R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-3094R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated