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Catalog #1: bsm-60709r-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-60709r-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1:THP-1, 2: Ramos, 3: A431, 4: Mouse \r\nbrain, 5: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 71 kDa\r\nObserved MW: 115, 19 kDa
Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60710R

Catalog #1: bsm-60710r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+ Human \u03b1-TNF \r\n(20ng\/ml, 30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): C6, (+): C6+ Calyculin A (100nM, \r\n30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 \r\nU\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline \r\nphosphatase (50 U\/ml) at 37\u2103 for 1 hour\r\nSection type: Formalin-fixed & Paraffin-embedded section \r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature \r\nSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60711R

Catalog #1: bsm-60711r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse \r\nheart\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 20 kDa\r\nObserved MW: 15 kDa
Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60712R
Tissue: Human Kidney\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor Tissue: Human Kidney\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60712R

Catalog #1: bsm-60712r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Tissue: Mouse kidney\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60713R
25 ug total protein per lane of various lysates (see on figure) probed with Calbindin monoclonal antibody, unconjugated (bsm-60713R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calbindin Monoclonal Antibody, Unconjugated(bsm-60713R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calbindin Monoclonal Antibody, Unconjugated(bsm-60713R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60713r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60713r-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-60713r-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-60713r-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-60713r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-60713r-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-60713r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-60713r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-60713r-bf350-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60713r-bf594-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60713r-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-60713r-bf555-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60713r-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60713r-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-60713r-bf405-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60713r-bf750-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60713r-apc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-60713r-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-60713r-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-60713r-bf488-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60713r-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-60713r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-60713r-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-60713r-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-60713r-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60713r-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-60714r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FGFR3 antibody (bsm-60715R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGFR3 Monoclonal Antibody, Unconjugated (bsm-60715R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGFR3 Monoclonal Antibody, Unconjugated (bsm-60715R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGFR3 Monoclonal Antibody, Unconjugated (bsm-60715R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60715r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60715r-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60715r-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60715r-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60715r-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60715r-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-60715r-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60715r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-60715r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60715r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60715r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60715r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60715r-bf647-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60715r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-60715r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60715r-bf680-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-680