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Catalog #1: bs-53088R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Whole cell extracts (40 \u03bcg) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.
HeLa cells were stained with HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC2 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
ChIP assays were performed using HeLa cells, HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 \u03bcg of HDAC2 (7G5) Monoclonal Antibody (Cat. No. C15200201) as described above. The IP\u2019d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer\u2019s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 3 (fig 2A and B), and in a two genomic regions surrounding the LMO4 positive control gene and the MAGEC1 gene (figure 2C and D).

Catalog #1: bsm-53026M

Applications: WB, ELISA, IF(ICC), dot-blot, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Whole cell extracts from HL60 and NB4 cells (40 \u03bcg) were analysed by Western blot using TET2 (6A1) Monoclonal Antibody (bsm-53017M), diluted 1:2,000 in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
IP was performed on 250 \u03bcg HL60 RIPA cell lysate using TET2 (6A1) Monoclonal Antibody (bsm-53017M) (lane 3) or an IgG negative control (lane 2). The input sample (25 \u03bcg RIPA lysate) was used as a positive control (lane 1). The samples were analysed by Western blot using TET2 (6A1) Monoclonal Antibody (bsm-53017M), diluted 1:2,000 in PBS containing 10% milk.

Catalog #1: bsm-53017M

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-53011M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Western blot was performed on whole cell (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H2Apan Polyclonal Antibody (bs-53140R). The antibody was diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H2Apan Polyclonal Antibody (bs-53140R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2Apan antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of H2Apan Polyclonal Antibody (bs-53140R) in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32,500.
ChIP assays were performed using HeLa cells, \r\nH2Apan Polyclonal Antibody (bs-53140R) and optimized PCR primer sets for qPCR. ChIP was performed with sheared chromatin from 1 million cells using the IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and EIF4A2 promoters, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53140R

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against MBD2 (bs-53058R), diluted 1:500 in BSA\/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against MBD2 (bs-53058R). By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:185,000.

Catalog #1: bs-53058R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-53067R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-53079R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Western blot was performed on 150 \u03bcg nuclear extract from either NIH3T3 or HeLa cells with the TIP5 antibody (bs-53052R), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween- 20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.
ChIP assays were performed using the TIP5 antibody (bs-53052R). Chromatin from HEK293T and NIH3T3 cells were formaldehyde cross-linked and sheared to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4\u00b0C with 100 \u03bcg sheared chromatin and either 5 \u03bcl of the TIP5 antibody or 5 \u03bcl IgG which was used as negative IP control. The IP\u2019d DNA was analyzed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. The figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalized to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

Catalog #1: bs-53052R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Western blot was performed on nuclear extracts from HeLa cells (40 \u03bcg) with the antibody against PADI4 (bs-53047R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against PADI4 (bs-53047R). The wells were coated with the peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 40,200.

Catalog #1: bs-53047R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53082R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53086R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-23146R

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Immunoprecipitation was performed on nuclear extracts from cells transfected with FLAG-tagged L3MBTL1 (L3MBTL1-F) with the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) and with an IgG negative control antibody. Western Blot was performed with an anti-FLAG antibody to demonstrate the presence of L3MBTL1-F in the input (lane 1), in the precipitated fraction (P) and in the supernatant (SN). These data show that L3MBTL1-F was efficiently precipitated by the L3MBTL1 antibody (lane 3 and 5), whereas it was not precipitated by the IgG negative control antibody (lane 2 and 4).
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE) using the Bioss antibody against L3MBTL1 (Cat. No. bs-53097R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 2). A molecular weight marker is shown in lane 1; the location of the protein of interest is indicated on the right.

Catalog #1: bs-53097R

Applications: WB, IP, IF

Reactivity: Human

Conjugate: Unconjugated

To determine the titer of the antibody, an ELISA was performed using a serial dilution of Bioss antibody directed against human DNMT3A (Cat. No. bs-53128R), crude serum and Flow Through, in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:500.
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 \u03bcg of DNMT3A Polyclonal Antibody (Cat. No. bs-53128R). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with the anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3).

Catalog #1: bs-53128R

Applications: WB, ELISA, IP, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Mouse muscle lysates probed with CRHR1 Polyclonal Antibody, Unconjugated (bs-10248R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse kidney lysates probed with CRHR1 Polyclonal Antibody, Unconjugated (bs-10248R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRHR1 Polyclonal Antibody, Unconjugated (bs-10248R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRHR1 Polyclonal Antibody, Unconjugated (bs-10248R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-10248R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Rat lung lysates probed with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10442R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Caspase-1 p20 Polyclonal Antibody(bs-10442R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10442R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10442R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10557R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-10551R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10576R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10592R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10603R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

MCF-7 lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Kindey lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human MCF-7 cell lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10764R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-12605R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-12613R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-12647R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-12690R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12740R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12780R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-12894R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13766R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-13791R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13807R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13808R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13836R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hepg2 lysates; Lane 2: A549 lysates probed with CELF3 Polyclonal Antibody, Unconjugated (bs-13830R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates ; Lane 5: Human SH-SY5Y cell lysates probed with CELF3 Polyclonal Antibody, Unconjugated (bs-13830R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13830R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13866R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13873R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13929R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13932R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13937R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13935R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-13969R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-14148R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver lysates probed with CYP2C18 Polyclonal Antibody, Unconjugated (bs-14150R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Mouse Liver lysates; Lane 2: Mouse Cerebrum ; Lane 3: HL60 cell lysates probed with CYP2C18 Polyclonal Antibody, Unconjugated (bs-14150R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-14150R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-14163R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-14164R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-14294R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated