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Primary Antibodies

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Catalog #1: bs-8165R-A680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-8165R-A750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-9877R-A594

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-9877R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: Biotin

Catalog #1: bs-9877R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: HRP

Catalog #1: bs-9877R-A350

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-9877R-A488

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 488

Antigen: bs-9877P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SW480 cell lysates probed with Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9877R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: Unconjugated

Catalog #1: bs-9877R-A555

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-9877R-A647

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-9877R-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: Cy3

Catalog #1: bs-9877R-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: Cy5

Catalog #1: bs-9877R-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: FITC

Catalog #1: bs-9877R-A750

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-9877R-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: Cy7

Catalog #1: bs-9877R-A680

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Horse, Others

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-10680R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5

Catalog #1: bs-10680R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-10680R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-10680R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-10680R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-10680R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-10680R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-10680R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-10680R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy3

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U-87MG lysates probed with NF-H Polyclonal Antibody, Unconjugated (bs-10680R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Tissue\/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NF-H) polyclonal Antibody, Unconjugated (bs-10680R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10680R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-10680R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy7

Catalog #1: bs-10680R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: FITC

Catalog #1: bs-10680R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-10680R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-33219M-A594

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-33219M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bsm-33219M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bsm-33219M-A488

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-33219M-A350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-33219M-A555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 555

BRL-3A cell lysates probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NIH\/3T3 Cell lysates; Lane 2: RAW246.7 Cell lysates; probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human Jurkatcell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Heart lysates; Lane 6: Rat Heart lysates; Lane 7: Mouse Skeletal muscle lysates; Lane 8: Rat Skeletal muscle lysates; Lane 9: Mouse Spleen lysates; Lane 10: Human MOLT-4 cell lysates probed with PI3K p85 Monoclonal Antibody, Unconjugated (bsm-33219M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33219M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-33219M-A647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-33219M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bsm-33219M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bsm-33219M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bsm-33219M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bsm-33219M-A680

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-33219M-A750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-0748R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-0748R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Lane 1:Mouse Cerebrum lysates probed with PTEN Polyclonal Antibody, Unconjugated (bs-0748R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PTEN Polyclonal Antibody(bs-0748R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PTEN) polyclonal Antibody, Unconjugated (bs-0748R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0748R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Lane 1: NCCIT; Lane 2: PC12; Lane 3: F9; Probed with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1\/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52262R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish

Conjugate: Unconjugated