Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies



×

Collection Menu

Catalog #1: bs-9990R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9990R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9990R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-9990R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-9990R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-9990R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-9991R-Biotin

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-9991R-HRP

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-9991R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9991R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-9991R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-9991R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-9991R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-9992R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9992R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9992R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9992R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-9992R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-9992R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-9992R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-9995R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9995R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9995R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9995R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-9995R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-9995R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-9995R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-9998R-Biotin

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-9997R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse Brain lysates probed with C4orf44 Polyclonal Antibody, Unconjugated (bs-9998R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Mouse brain lysates probed with C4orf44 Polyclonal Antibody, Unconjugated (bs-9998R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse brain lysates probed with C4orf44 Polyclonal Antibody, Unconjugated (bs-9998R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9998R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9998R-HRP

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-9998R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-9998R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-9998R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-9998R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-9999R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0387m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-TNF-alpha Polyclonal Antibody, FITC conjugated (bsm-0387M-FITC) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded:human colon labeled with Anti-TNF-Alpha Polyclonal Antibody, Unconjugated (bsm-0387M), followed by conjugation to the secondary antibody and DAB staining
4% Paraformaldehyde-fixed THP-1(LPS treated (100 ng\/ml, 16 h) and Brefeldin A treated (5 \u00b5g\/ml, 4 h)) (H) cell; Antibody incubation with (TNF alpha) monoclonal Antibody, unconjugated (bsm-0387M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Blank control:THP-1. Primary Antibody (green line): Mouse Anti-TNF alpha antibody (bsm-0387M) Dilution: 1ug\/Test; Secondary Antibody : Goat anti-mouse IgG-FITC Dilution: 0.5ug\/Test. Protocol The cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bsm-0387m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0387m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0387m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0387m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-0387m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-0387m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BrdU (A7)) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-0917m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-0917m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-0917m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-0917m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-0917m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5