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phospho-STAT3 (Ser727) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-STAT3 (Ser727)) Polyclonal Antibody, Unconjugated (bs-25609R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-STAT3 (Ser727)) Polyclonal Antibody, Unconjugated (bs-25609R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-STAT3 (Ser727)) Polyclonal Antibody, Unconjugated (bs-25609R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-STAT3 (Ser727)) Polyclonal Antibody, Unconjugated (bs-25609R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • ELISA
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-25609R
Product Name phospho-STAT3 (Ser727) Polyclonal Antibody
Applications ELISA, IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Sheep, Pig, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Ser727
Modification Site Ser727
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6774
Swiss Prot P40763
Subcellular location Cytoplasm, Nucleus
Synonyms STAT3 (phospho S727); p-STAT3 (phospho S727); STAT3(Phospho Ser727); STAT3(Phospho S727); p-STAT3(Ser727); Acute Phase Response Factor; APRF; DNA binding protein APRF; Signal Transductor and Activator of Transcription 3; STAT 3; STAT3_HUMAN.
Application Dilution
ELISA =1:5000-10000
IHC-P IHC-P=1:100-500

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