Smad3 (Ser423 + Ser425) Antibody, ALEXA FLUOR® 350 Conjugated

Applications

WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Pig

Predicted Reactivity

Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-3425R-A350
Product Name	Smad3 (Ser423 + Ser425) Antibody, ALEXA FLUOR® 350 Conjugated
Applications	WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	These phosphorylation sites are homologous across the species listed.
Reactivity	Human, Mouse, Rat, Pig
Predicted Reactivity	Dog, Cow, Horse, Chicken
Specifications
Conjugation	ALEXA FLUOR® 350
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]
Modification Site	Ser423 + Ser425
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	4088
Swiss Prot	P84022
Subcellular location	Cytoplasm, Nucleus
Synonyms	LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Background	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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