PLAUR Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PLAUR) Monoclonal Antibody, Unconjugated (ascites of bsm-33874M ) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PLAUR) Monoclonal Antibody, Unconjugated (ascites of bsm-33874M ) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.\r\n\r\n

Hela cells were incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PLAUR Monoclonal Antibody(bsm-33874M)at 1:20 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).