Vitamin D Binding protein (56C9) Monoclonal Antibody

Flow cytometric analysis of HepG2 cells with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Human lung lysates probed with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Human lung lysates probed with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-52884R
Product Name	Vitamin D Binding protein (56C9) Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Vitamin D Binding protein aa 250-350
Clonality	Monoclonal
Clone #	56C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2638
Swiss Prot	P02774
Synonyms	Gc protein-derived macrophage activating factor, Group-specific component, DBP, VDB, Gc-MAF, Gc, DBP-maf
Background	Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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