XBP1 (2C5) Monoclonal Antibody

Flow cytometric analysis of 293T cells with XBP1 (2C5) Monoclonal Antibody (bsm-52898R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: HepG2 cells lysates; Lane 2: 293T brain cells lysates; Probed with XBP1 (2C5) Monoclonal Antibody (bsm-52898R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 cells lysates; Lane 2: 293T brain cells lysates; Probed with XBP1 (2C5) Monoclonal Antibody (bsm-52898R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-52898R
Product Name	XBP1 (2C5) Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human XBP1 aa 100-200
Clonality	Monoclonal
Clone #	2C5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7494
Swiss Prot	P17861
Synonyms	X-box-binding protein 1, Tax-responsive element-binding protein 5, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, luminal form, XBP-1, TREB-5
Background	Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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