Alpha B Crystallin (1B12) Monoclonal Antibody

$Lane 1: Human Skeleton Muscle; Lane 2: Mouse Heart; Probed with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Skeleton Muscle; Lane 2: Mouse Heart; Probed with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Embryonic Skeletal Muscle tissue incubated with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Embryonic Skeletal Muscle tissue incubated with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Alpha B Crystallin (1B12) Monoclonal Antibody (bsm-54032R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Rat

Overview
Catalog #	bsm-54032R
Product Name	Alpha B Crystallin (1B12) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	1B12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1410
Swiss Prot	P02511
Synonyms	Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5, CRYAB, CRYA2, HSPB5
Background	Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into _, _ and _ families, and the _- and _-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. _-crystallins consist of three gene products, _A-, _B- and _C-crystallin, which are members of the small heat shock protein family (HSP 20). _-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, _-crystallins do not renature these proteins. Expression of _A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human _B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of _B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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