TNPO3 (11F2) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2: HL-60 Cells; Lane 3: Mouse Placenta; Probed with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: HL-60 Cells; Lane 3: Mouse Placenta; Probed with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with TNPO3 (11F2) Monoclonal Antibody (bsm-54411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
ELISA
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54411R
Product Name	TNPO3 (11F2) Monoclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human TNPO3 aa 800-950.
Clonality	Monoclonal
Clone #	11F2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	23534
Swiss Prot	Q9Y5L0
Subcellular location	Cytoplasm, Nucleus
Synonyms	Transportin-3; Importin-12; Transportin-SR; Imp12; TRN-SR; TNPO3; IPO12
Background	TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins such as the splicing factors SFRS1 and SFRS2 by recognizing phosphorylated RS domains. TNPO3 has also been shown to be involved in HIV-1 infection, apparently through interaction with the HIV-1 capsid protein. Two transcript variants encoding different isoforms as well as a noncoding transcript have been found for this gene
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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