Kininogen 1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen 1 Monoclonal Antibody, Unconjugated (bsm-54645R) at 1:100 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen 1 Monoclonal Antibody, Unconjugated (bsm-54645R) at 1:100 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen 1 Monoclonal Antibody, Unconjugated (bsm-54645R) at 1:100 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen 1 Monoclonal Antibody, Unconjugated (bsm-54645R) at 1:100 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54645R
Product Name	Kininogen 1 Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Kininogen 1 aa 500-644.
Clonality	Monoclonal
Clone #	4G7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3827
Swiss Prot	P01042
Subcellular location	Secreted
Synonyms	Alpha-2-thiol proteinase inhibitor antibody, BDK antibody, BK antibody, Bradykinin antibody, Bradykinin included antibody, Fitzgerald factor antibody, FLAUJEAC FACTOR antibody, High molecular weight kininogen antibody, HMWK antibody, Ile-Ser-Bradykinin antibody, Kallidin I antibody, Kallidin II antibody, KNG antibody, KNG1 antibody, KNG1_HUMAN antibody, Low molecular weight growth-promoting factor antibody, WILLIAMS FACTOR antibody, Williams-Fitzgerald-Flaujeac factor antibody
Background	This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC

Applications

Reactivity

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