Tri-Methyl-Histone H3 (Lys4) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7, rH3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7, rH3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

Applications

Reactivity

Human
Mouse
Rat
Monkey

Overview
Catalog #	bsm-60130M
Product Name	Tri-Methyl-Histone H3 (Lys4) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat, Monkey
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	H4F8
Isotype	IgG
Concentration	1mg/ml
Purification	Protein G coupled with the antigen peptide containing H3K4me3.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Application Dilution
WB	1:300-5000

Applications

Reactivity

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