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Unconjugated Primary Antibodies



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Catalog #1: bs-24804R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (muscle Actin) Polyclonal Antibody, Unconjugated (bs-13947R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with muscle Actin polyclonal antibody, unconjugated (bs-13947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-13947r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.
To determine the titer, an ELISA was performed using a serial dilution of the Ash2 antibody (bs-53055R). The wells were coated with the peptides used for immunization of the host rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.

Catalog #1: bs-53055R

Applications: WB, ELISA, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-53087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53070R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53142R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53141R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51079R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51067R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51058R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51068R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51087R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51088R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51070R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51146R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51080R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51141R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51118R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51093R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51104R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51084R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51128R

Applications: WB, ELISA, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51086R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51047R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51077R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51140R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51089R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51090R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51081R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51145R

Applications: ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51144R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51142R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51137R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51135R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51132R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51131R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51126R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Yeast

Conjugate: Unconjugated

Catalog #1: bs-51122R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Rat, Others, Zebrafish, Drosophila

Conjugate: Unconjugated

Catalog #1: bs-51121R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Mouse, Yeast

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K36me3 Polyclonal Antibody H3K36me3 (bs-51116R) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk.
A Dot Blot analysis was performed to test the cross reactivity of H3K36me3 Polyclonal Antibody(bs-51116R) with peptides containing other H3 and H4 modifications and unmodified sequence. 0.2-100 pmol of peptide containing histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The result demonstrates high specificity of the antibody for the modification of interest.

Catalog #1: bs-51116R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Zebrafish, Drosophila

Conjugate: Unconjugated

Catalog #1: bs-51114R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-51111R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Yeast

Conjugate: Unconjugated

Catalog #1: bs-51109R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51108R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51106R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51105R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51074R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated