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Formalin-fixed and paraffin embedded rat brain labeled with Anti Nur77 Polyclonal Antibody (bs-3513R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Sample: <br>\r\nLane 1: Human Jurkat cell lysates<br>\r\nLane 2: Human HeLa cell lysates<br>\r\nPrimary: Anti-Nur77 (bs-3513R) at 1\/1000 dilution<br>\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution<br>\r\nPredicted band size: 66 kDa<br>\r\nObserved band size: 73 kDa<br>\r\nLot: AI11268723\r\n

Catalog #1: bs-3513R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-LC3B\/MAP LC3\u03b2\/MAP1A Polyclonal Antibody, Unconjugated (bs-2912R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human LC3B protein, N-His(bs-42305P) probed with LC3B Polyclonal Antibody, Unconjugated (bs-2912R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-2912r

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-2601R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded chicken ileum labeled with Anti-CD44V6 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:300 followed by conjugation to the secondary antibody
Rat lung lysate probed with Anti CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 80kD. Observed band size: 80kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44 Polyclonal Antibody(bs-2507R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2507R

Applications: ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2485R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-2284R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated \\tbs-20817R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20817R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (bs-2043R-Cy3) at 1:200 for 60 minutes at 37C.
PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody (bs-2043R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Paraformaldehyde-fixed, paraffin embedded human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2043R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PEG3 Polyclonal Antibody, Unconjugated(bs-1870R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PEG3 Polyclonal Antibody(bs-1870R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (bs-1870R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (bs-1870R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1870R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC9A Polyclonal Antibody, Unconjugated (bs-13621R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC9A Polyclonal Antibody, Unconjugated (bs-13621R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-13621R

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Human 293T cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A549 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FAK) polyclonal Antibody, Unconjugated (bs-1340R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (bs-1340R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1340R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13374R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-12998r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-GPA33 Polyclonal Antibody, Unconjugated (bs-1226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-GPA33 Polyclonal Antibody, Unconjugated (bs-1226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1226R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: mouse lung lysates probed with \\tTAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nTAZ Polyclonal Antibody(bs-12367R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-12367R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11745R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11460R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-10341R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10407R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Independently Validated Antibody, image provided by Science Direct, badge number 028752. Western blot analysis of mouse brain and liver extracts using NK2 Homeobox 1 (bs-0826R NKX2-1) antibody, 1:500 dilution. NKX2-1 is present in the positive control sample (brain) and absent from the negative control sample (liver). The predicted and observed position of NKX2-1 is at around 38kDa.
PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody (bs-0826R) at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min. This data was generously submitted by an end user as part of our Bioss Discovery program.
Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (bs-0826R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Mouse Thyroid gland Lysates; Lane 2: Rat Thyroid gland Lysates. Probed with TTF1\u00a0 polyclonal Antibody, unconjugated (bs-0826R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0826R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0699R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney tissue labeled with Anti-Acetyl-Histone H1b(K53) Polyclonal Antibody (bs-0931R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spleen tissue labeled with Anti-Acetyl-Histone H1b(K53) Polyclonal Antibody (bs-0931R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H1 (Lys52) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H1 (Lys52) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0931R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U87MG Cell lysates probed with FSCN1(Ser39) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibodyincubation at 1:20000 for 60 min at 37˚C.\r\n
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FSCN1(Ser39) Polyclonal Antibody(bs-0772R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (bs-0772R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0772R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2 Polyclonal Antibody(bs-0908R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human K562 cell lysates; Lane 2: Mouse BV-2 cell lysates probed with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0908R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (bs-0520R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0520R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with progesterone receptor Antibody(bs-0111R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Progesterone Receptor Antibody(bs-0111R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-0111R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0111R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

\"Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-HSP-70 Polyclonal Antibody (bs-0126R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n\"
Generously provided by Merlin Levine from National Institutes of Health as part of the Bioss Discovery Program. Mouse brain labeled with Anti-HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:100 overnight. The secondary antibody was AlexaFluor 555 goat anti mouse IgG1 used at 1:250 for one hour.
Mouse bladder lysates probed with HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0126R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (alpha 1 Antitrypsin) Polyclonal Antibody, Unconjugated (bs-0096R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining

Catalog #1: bs-0096R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0427R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0155R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human uterus labeled with Anti-Calretinin\/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Calretinin\/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates probed with Calretinin Polyclonal Antibody, Unconjugated (bs-0062R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0062R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-20746R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-20746R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-20746R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-20746R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20746R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse cerebllumlysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-23376R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Flow cytometric analysis of Jurkat cells with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52396r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded Mouse Placenta tissue labeled with CD274 Polyclonal Antibody, Unconjugated (bs-10159R) at 1:200 followed by incubation with a secondary antibody, DAB and counter-staining
Mouse Spleen Cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1 X PBS\/2%BSA\/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD274 Polyclonal Antibody, PE-Conjugated (bs-10159R-PE) at 1:100 for 30 minutes at room temp. The graph compares the primary antibody (green) to unstained cells (dark blue), and isotype control (orange; bs-0295P-PE).
Lane 1: Du145 cell lysates; Lane 2: Lovo cell lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Muscle lysates ; Lane 5: Rat Heart lysates probed with CD274 Polyclonal Antibody, Unconjugated (bs-10159R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD274 Polyclonal Antibody(bs-10159R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10159R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart lysates probed with LCLAT1 Polyclonal Antibody, Unconjugated (bs-18190R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with LCLAT1 Polyclonal Antibody, Unconjugated (bs-18190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-18190R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0966M

Applications: WB, IF(ICC)

Reactivity: Tags

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CCDC92) Polyclonal Antibody, Unconjugated (bs-8142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CCDC92) Polyclonal Antibody, Unconjugated (bs-8142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-8142R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-8033R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human LOVO cell lysates probed with APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6587R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6640R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6133R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded smooth muscle of rat colon labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat brain lysates probed with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD
Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #1: bs-5882R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5493R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-4964R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-IGF I Polyclonal Antibody, Unconjugated (bs-4588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: IGF1 Recombinat Protein lysates; Lane 2: Contro(-) lysates probed with IGF 1 Polyclonal Antibody, Unconjugated (bs-4588R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-4588R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-PER2\/Period circadian protein 2 Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-PER2\/Period circadian protein 2 Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3927R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3971R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated