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Unconjugated Primary Antibodies



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Formalin-fixed and paraffin embedded rat kidney labeled with Anti PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4\u00b0C, DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PNMT) polyclonal Antibody, Unconjugated (bs-3912R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3912R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-SYN1\/Synapsin 1 Polyclonal Antibody, Unconjugated (bs-3501R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Lane 1: HL60 cell lysates probed with Synapsin 1 Polyclonal Antibody, Unconjugated (bs-3501R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3501R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIP2 Polyclonal Antibody, Unconjugated (bs-3546R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates probed with RIP2 Polyclonal Antibody, Unconjugated (bs-3546R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3546R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SIAH1 Polyclonal Antibody, Unconjugated (bs-3596R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with SIAH1 Polyclonal Antibody, unconjugated (bs-3596R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with SIAH1 Antibody(bs-3596R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-3596R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with Talin Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Talin Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3619R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Phospho-c-Kit(Tyr721) Polyclonal Antibody, Unconjugated (bs-3242R) followed by conjugation to the secondary antibody and DAB staining
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with c-Kit(Tyr721) Polyclonal Antibody(bs-3242R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Kit(Tyr721) Polyclonal Antibody, Unconjugated (bs-3242R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3242R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-Tau protein(Ser396) Polyclonal Antibody, Unconjugated (bs-3446R) at 1:200 followed by conjugation to the secondary antibody
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau protein(Ser396) Polyclonal Antibody, Unconjugated (bs-3446R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3446R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Doublecortin (Ser47) Polyclonal Antibody, Unconjugated (bs-3113R) at 1:400 overnight at 4\u00b0C, DAB staining.
U251 cell lysates probed with Doublecortin (Ser47) Polyclonal Antibody, Unconjugated (bs-3113R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Human HepG2 Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 0.2% PBST for 30min at room temperature. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with Doublecortin (Ser47) Polyclonal Antibody, Unconjugated (bs-3113R) at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-FITC (bs-0295G-FITC), was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (black), secondary only (blue) and isotype control (bs-0295P; orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with Doublecortin (Ser47) Polyclonal Antibody, Unconjugated (bs-3113R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3113R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Noggin Polyclonal Antibody, Unconjugated (bs-2975R) at 1:200 overnight at 4\u00b0C, followed by a conjugated
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Noggin Polyclonal Antibody, Unconjugated (bs-2975R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Noggin Polyclonal Antibody, Unconjugated (bs-2975R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2975R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-HES1 Polyclonal Antibody, Unconjugated (bs-2972R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HES1 Polyclonal Antibody, Unconjugated (bs-2972R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HES1 Antibody(bs-2372R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HES1) polyclonal Antibody, Unconjugated (bs-2972R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2972R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2966R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2709R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

L1 rat lung lysates, L2 rat brain lysates probed (bs-2930R) Anti-Frizzled-5 Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 62kD.
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Frizzled 5\/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Frizzled 5\/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
K562 cell lysates probed with Rabbit Anti-Frizzled 5\/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-2930R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2679R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-PDE5A Polyclonal Antibody, Unconjugated(bs-2349R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: Human HT1080 cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with PDE5A Polyclonal Antibody, Unconjugated (bs-2349R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2349R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreatic cancer labeled with Anti-CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human Jurkat cell lysates probed with CRIM1 Polyclonal Antibody, Unconjugated (bs-2034R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2034R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1985R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

RSC96 cells probed with DRD3 Polyclonal Antibody, Unconjugated (bs-1743R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with DRD3 Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Brain lysates; Lane 2: Rat Brain lysates probed with Dopamine D3 receptor Polyclonal Antibody, Unconjugated (bs-1743R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1743R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti FDC-SP\/ADC Polyclonal Antibody, Unconjugated (bs-1766R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse lymphoid tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FDC-SP Polyclonal Antibody, Unconjugated (bs-1766R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining. \r\n\r\n

Catalog #1: bs-1766R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse uterus lysates probed with GPAT4 Polyclonal Antibody, Unconjugated (bs-15587R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Human A549 cell lysates probed with GPAT4 Polyclonal Antibody, Unconjugated (bs-15587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-15587R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-15552R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Placenta lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human MOLT-4 cell lysates probed with TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1444R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti PAFAH2\/Lp-PLA2 Polyclonal Antibody, Unconjugated (bs-1451R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded Rabbit Cartoid labeled with Anti-PAFAH2\/Lp-PLA2 Polyclonal Antibody, Unconjugated (bs-1451R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat spleen labeled with Anti-PAFAH Polyclonal Antibody, Unconjugated(bs-1451R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1451R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13628R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Human A549 and A431 cell lysates probed with GPR110 Polyclonal Antibody, unconjugated (bs-13513R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse kidney cells probed with \\tGPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-13513R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-13754R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13522r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WDFY1\/2 Polyclonal Antibody, Unconjugated (bs-13169R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human U87MG cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U2os cell lysates; Lane 4: Human A431 cell lysates probed with FENS1 Polyclonal Antibody, Unconjugated (bs-13169R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane2: Rat Cerebrum tissue lysates; Lane 3: Rat Skin tissue lysates; Lane 4: Human Huvec cell lysates ; Lane 5: Human HepG2 cell lysates ; Lane 6: Human THP-1 cell lysates probed with FENS1 Polyclonal Antibody, Unconjugated (bs-13169R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13169R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse testis lysates; Lane 2: Mouse thymus lysates probed with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bs-13119r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-13185R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1327R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1296R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-SFRP1 Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SFRP1 Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1303R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12145R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12017R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti ROCK2 Polyclonal Antibody, Unconjugated (bs-1205R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti ROCK2 Polyclonal Antibody, Unconjugated (bs-1205R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 161kD. Observed band size: 161kD
Lane 1: Mouse Heart Lysates; Lane 2: Mouse Skeletal muscle Lysates. Probed with ROCK2 polyclonal Antibody, unconjugated (bs-1205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1205R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Spinal cord lysates; Lane 3: Mouse Stomach lysates; Lane 4: Rat Cerebellum lysates ; Lane 5: Rat Spinal cord lysates probed with 5HT3A Receptor Polyclonal Antibody, Unconjugated (bs-12051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with 5HT3A Receptor\/SR-3A Polyclonal Antibody, Unconjugated (bs-12051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12051R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12046R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11884r

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1184R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CLN8 Polyclonal Antibody, Unconjugated (bs-11715R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.
Hela cell lysates probed with CLN8 Polyclonal Antibody, Unconjugated (bs-11715R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CLN8) Polyclonal Antibody, Unconjugated (bs-11715R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CLN8) Polyclonal Antibody, Unconjugated (bs-11715R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-11715R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti FOXD3 Polyclonal Antibody, Unconjugated (bs-11518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti FOXD3 Polyclonal Antibody, Unconjugated (bs-11518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FOXD3) polyclonal Antibody, Unconjugated (bs-11518R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11518R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11529R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LHX2 Polyclonal Antibody, Unconjugated (bs-11200R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human HeLa cell lysates probed with LHX2 Polyclonal Antibody, Unconjugated (bs-11200R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11200R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1051R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-CSIG Polyclonal Antibody, Unconjugated (bs-0793R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti CSIG Polyclonal Antibody, Unconjugated (bs-0793R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 54-60kD. Observed band size:54kD.\\n
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (bs-0793R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0793R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0787P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IFN-beta Polyclonal Antibody, Unconjugated (bs-0787R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human endometrial cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFN-beta Polyclonal Antibody, Unconjugated (bs-0787R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFN-beta Polyclonal Antibody, Unconjugated (bs-0787R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0787R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Human A549 cell lysates probed with Rabbit Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRP1 Polyclonal Antibody(bs-0657R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0657R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated